Using a combination of different methods, we investigate signaling processes in living cells. We use patch-clamp and live-cell imaging techniques (combined with confocal and wide-field microscopy) to investigate physiological processes at the single-cell and network level. In anatomical studies, we employ classical histology and CLARITY. Classical confocal microscopy as well as light sheet microscopy (Leica SP8 DLS, UltraMicroscope Blaze™, Miltenyi) and 2-photon microscopy are available for the analyses. Molecular biology (PCR, ISH) and behavioral studies support our investigations.